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原价:
¥2,200.00
现价:
¥1,850.00
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LNCap-C4-2B人前列腺癌细胞ATCC:CRL-3315

基本信息

货号 C1287
名称 LNCap-C4-2B人前列腺癌细胞ATCC:CRL-3315
规格 1 x 10^6 cells/vial

产品详情

英文名称

C4-2B; C4-2 B; C4-2 Bone metastatic

描述

Derivative subline of human prostate cancer LNCaP-derived C4-2 cells.

Origin: The human prostatic carcinoma cell line LNCaP (previously described) was co-inoculated into an athymic male nude mouse with human fibroblasts derived from an osteosarcoma. The nude mouse host was castrated after 8 weeks incubation. A tumor specimen was excised after a total of 12 weeks. The C4 cell line constitutes the in vitro cultured subline grown from the murine host’s tumor. When the C4 sub-line was subsequently co-inoculated with MS osteosarcoma fibroblasts in a castrated athymic male nude mouse host for another 12 weeks by the same protocol described above. Prostatic epithelial cells cultured from the resultant tumor in this host constituted the C4-2 subline.The STR identification in this library is correct.

种属

组织来源

前列腺

形态

上皮样

生长方式

贴壁

无菌检测

细菌、酵母、支原体检测匀为阴性

病原体检查

HIV、乙型肝炎、丙型肝炎检查均为阴性

运输方式

复苏发货或干冰冷冻发货

安全性

所有肿瘤和病毒转染的细胞均视为有潜在的生物危害性,必须在二级生物安全台内操作,并请注意防护。

培养

推荐WHELAB自配培养基:

RPMI-1640货号:M0200+ 10%胎牛血清 +2% GermClean

配套完全培养基:货号:M0201

温度:37

气相:95%空气,5%二氧化碳

保存

冻存条件:细胞保存液(货号:G0200

保存条件:液氮存储

供应限制

仅供科学研究之用。