英文名称 | HT 29; HT29 |
描述 | In 1964, the HT-29 cell line was isolated from the primary tumor by J. Fogh using a transplant culture method and F12 culture solution containing 15% FBS. Recently, cultured cells from established strains were cultured with mcCoy's 5a culture solution containing the same serum. This cell line is tumoritic in nude mice and can also be tumorigened in steroid-treated gophers. This cell line synthesizes IgA, CEA, a protein-bound secretion component of TGFβ and mucin. The STR identification in this library is correct. |
种属 | 人 |
组织来源 | 结直肠腺癌 |
形态 | 上皮细胞样 |
生长方式 | 贴壁 |
无菌检测 | 细菌、酵母、支原体检测匀为阴性 |
病原体检查 | HIV、乙型肝炎、丙型肝炎检查均为阴性 |
运输方式 | 复苏发货或干冰冷冻发货 |
安全性 | 所有肿瘤和病毒转染的细胞均视为有潜在的生物危害性,必须在二级生物安全台内操作,并请注意防护。 |
培养 | 推荐WHELAB自配培养基: ※ McCoy's 5A(货号:M0600)+ 10%胎牛血清 ※ 配套完全培养基:(货号:M0601) 温度:37℃ 气相:95%空气,5%二氧化碳 |
保存 | 冻存条件:细胞保存液(货号:G0200) 保存条件:液氮存储 |
供应限制 | 仅供科学研究之用。 |
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HT-29人结肠癌细胞ATCC:HTB-38
基本信息
货号 | C1007 |
名称 | HT-29人结肠癌细胞ATCC:HTB-38 |
规格 | 1 x 10^6 cells/vial |