英文名称 | MCF-12A |
描述 | The MCF-12A cell line is a non-tumorigenic epithelial cell line established from tissue taken at reduction mammoplasty from a nulliparous patient with fibrocystic breast disease that contained focal areas of intraductal hyperplasia. The line was produced by long term culture in serum free medium with low Ca++ concentration. MCF-12A was derived from adherent cells in the population. |
种属 | 人 |
组织来源 | 乳腺 |
形态 | 上皮样 |
生长方式 | 贴壁 |
无菌检测 | 细菌、酵母、支原体检测匀为阴性 |
病原体检查 | HIV、乙型肝炎、丙型肝炎检查均为阴性 |
运输方式 | 复苏发货或干冰冷冻发货 |
安全性 | 所有肿瘤和病毒转染的细胞均视为有潜在的生物危害性,必须在二级生物安全台内操作,并请注意防护。 |
培养 | 推荐WHELAB自配培养基: ※DMEM+F12+20 ng/ml 人表皮生因子+100 ng/ml 霍乱毒素+0.01 mg/ml bovine insulin+500 ng/ml hydrocortisone+5%马血清 温度:37℃ 气相:95%空气,5%二氧化碳 |
保存 | 冻存条件:细胞保存液(货号:G0200) 保存条件:液氮存储 |
供应限制 | 仅供科学研究之用。 |
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MCF-12A人乳腺癌细胞ATCC:CRL-7762
基本信息
货号 | C1751 |
名称 | MCF-12A人乳腺癌细胞ATCC:CRL-7762 |
规格 | 1 x 10^6 cells/vial |