PA317小鼠成纤维细胞ATCC:CRL-9078

英文名称

pA317

描述

The PA317 cell line is derived from TK-NIH/3T3 cells and constructed by a joint transfection of the packaging structure DNA (pPAM3) in pBR322 and the thymidine kinase gene of herpes simplex virus in pBR322. Retroviral vectors are introduced into these cells by infection or transfection, resulting in biphilic vector particles that can infect many mammalian cells. This cell-generated virus particle has been successfully used to introduce genes into humans. Possibly due to the loss of DNA transferred to build this strain of cells, the percentage of PA317 cells capable of packaging retroviral vectors decreases with passage.After selection, cells should be cultured in HT medium (0.03 mM times xanthine, 0.02 mM thymidine) for 4 days to dilute residual methotrexate. Cells shipped from the ATCC have been selected in medium containing 0.03 mM hypoxanthine, 0.001 mM amethopterin (methotrexate), and 0.02 mM thymidine prior to freezing, and should be grown in HT medium for 4 days after receipt. Thereafter the cells can be stabilized for at least 1 month without selection.

种属

小鼠

组织来源

胚胎

形态

成纤维细胞

生长方式

贴壁

无菌检测

细菌、酵母、支原体检测匀为阴性

病原体检查

HIV、乙型肝炎、丙型肝炎检查均为阴性

运输方式

复苏发货或干冰冷冻发货

安全性

所有肿瘤和病毒转染的细胞均视为有潜在的生物危害性，必须在二级生物安全台内操作，并请注意防护。

培养

推荐WHELAB自配培养基：

※ DMEM高糖 （货号M0100）+ 10%胎牛血清

※ 配套完全培养基：（货号：M0101）

温度：37℃

气相：95%空气，5%二氧化碳

保存

冻存条件：细胞保存液（货号：G0200）

保存条件：液氮存储

供应限制

仅供科学研究之用。