PK136小鼠X小鼠ATCC; HB-191

英文名称

PK-136

描述

Animals were immunized with spleen and bone marrow cells from CE mice. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody is specific for NK cells and is cytotoxic in the presence of complement.The pattern of reactivity of the antibody with cells of various strains of mice matches the pattern of conventional NK 1.1 antisera.Tested and found negative for ectromelia virus (mousepox).

种属

小鼠

组织来源

hybridoma: b lymphocyte

形态

Lymphoblast-like

生长方式

Suspension

无菌检测

细菌、酵母、支原体检测匀为阴性

病原体检查

HIV、乙型肝炎、丙型肝炎检查均为阴性

运输方式

复苏发货或干冰冷冻发货

安全性

所有肿瘤和病毒转染的细胞均视为有潜在的生物危害性，必须在二级生物安全台内操作，并请注意防护。

培养

The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:

fetal bovine serum to a final concentration of 10%（货号：M0701）

1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.

保存

冻存条件：细胞保存液（货号：G0200）

保存条件：液氮存储

供应限制

仅供科学研究之用。